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課程名稱 |
基因科技學
CENE TECHNOLOGY |
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開課學期 |
95-2 |
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授課對象 |
學程 幹細胞與再生醫學學程 |
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授課教師 |
蔡懷楨 |
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課號 |
MCB5009 |
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課程識別碼 |
B43 U1200 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期二5,6(12:20~14:10) |
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上課地點 |
生科3A |
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備註 |
限學士班三年級以上
總人數上限：60人 |
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Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/952GT |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
GENE TECHNOLOGY (B43 U1200)
Tue 1:20-3:10 pm, Rm LSB 3A, (Spring, 2007)
Huai-Jen Tsai, IMCB, 3366-2487; hjtsai@ntu.edu.tw
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課程目標 |
1. Feb. 27 Ch. 1 Why gene cloning and DNA analysis are important?
Ch. 2 Vehicles for gene cloning: plasmids and bacteriophages
2. Mar. 6 Ch. 3 Purification of DNA from living cells
Ch. 4 Manipulation of purified DNA
3. Mar. 13 Ch. 5 Introduction of DNA into living cells
4. Mar. 20 Ch. 6 Cloning vectors for E. coli
5. Mar. 27 Ch. 7 Cloning vectors for eukaryotes
6. Apr. 3 Ch. 8 How to obtain a clone of a specific gene
7. Apr. 10 Ch. 9 PCR
Ch. 10 Studying gene location and structure
8. Apr. 17 Midterm Exam
9. Apr. 24 Ch. 11 Studying gene expression and function
10. May 1 Ch. 12 Studying genomes
11. May 8 Ch. 13 Purification of protein from cloned genes
12. May 15 Ch. 14 Gene cloning and DNA analysis in medicine
Ch. 15 Gene cloning and DNA analysis in agriculture (I)
13. May 22 Ch. 15 Gene cloning and DNA analysis in agriculture (II)
Ch. 16 Gene cloning and DNA analysis in forensic science
14. May 29 Gene transfer technology: mice
15. June 5 Gene transfer technology: model fish
16. June 12 Microarray, Real-time PCR
17. June 19 Holiday
18. June 26 Final Exam
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課程要求 |
教科書：T. A. Brown, 2001, Gene Cloning (4th edition)為主
成績評量：期中考或（及）期末考；繳交學期報告為optional. |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
1) Brown, 2006, Gene Cloning and DNA Analysis (5th edition), Blackwell Science
2) Reece, 2003, Analysis of Genes and Genomes, John Wiley & Sons
3) Barnum, 2005, Biotechnology (2nd edition), Thomson
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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第1週 |
2/27 |
Ch. 1 Why gene cloning and DNA analysis are important?
Ch. 2 Vehicles for gene cloning: plasmids and bacteriophages
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第2週 |
3/06 |
Ch. 3 Purification of DNA from living cells
Ch. 4 Manipulation of purified DNA
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第3週 |
3/13 |
Ch. 5 Introduction of DNA into living cells |
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第4週 |
3/20 |
Ch. 6 Cloning vectors for E. coli |
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第5週 |
3/27 |
Ch. 7 Cloning vectors for eukaryotes |
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第6週 |
4/03 |
Ch. 8 How to obtain a clone of a specific gene |
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第7週 |
4/10 |
Ch. 9 PCR
Ch. 10 Studying gene location and structure
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第8週 |
4/17 |
Midterm Exam |
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第9週 |
4/24 |
Ch. 11 Studying gene expression and function |
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第10週 |
5/01 |
Ch. 12 Studying genome |
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第11週 |
5/08 |
Ch. 13 Purification of protein from cloned genes |
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第12週 |
5/15 |
Ch. 14 Gene cloning and DNA analysis in medicine
Ch. 15 Gene cloning and DNA analysis in agriculture (I)
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第13週 |
5/22 |
Ch. 15 Gene cloning and DNA analysis in agriculture (II)
Ch. 16 Gene cloning and DNA analysis in forensic science
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第14週 |
5/29 |
Gene transfer technology: mice |
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第15週 |
6/05 |
Gene transfer technology: model fish |
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第16週 |
6/12 |
Microarray, Real-time PCR |
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第17週 |
6/19 |
Holiday |
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第18週 |
6/26 |
Final Exam |
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第19週 |
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term paper |
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